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                            網(wǎng)站首頁(yè)產(chǎn)品展示酶聯(lián)免疫ELISA試劑盒人的ELISA > 96T/48T人胰島素自身抗體(IAA)酶免ELISA試劑盒
                            人胰島素自身抗體(IAA)酶免ELISA試劑盒

                            人胰島素自身抗體(IAA)酶免ELISA試劑盒

                            產(chǎn)品型號(hào): 96T/48T

                            所屬分類(lèi):人的ELISA

                            產(chǎn)品時(shí)間:2024-08-13

                            簡(jiǎn)要描述:人胰島素自身抗體(IAA)酶免ELISA試劑盒價(jià)格公道、*,售后服務(wù)完整,并提供免費(fèi)代檢測(cè)服務(wù)!需要產(chǎn)品說(shuō)明書(shū)及其它詳細(xì)信息請(qǐng)直接與我們。

                            詳細(xì)說(shuō)明:

                            Drug Names

                            Generic NameHuman insulin autoantibodies IAA ELISA Kit.

                            Purpose

                            This kit allows for the determination of IAA concentrations in Human serum, blood plasma, and other biological fluids.

                            Principle of the assay

                            The kit assay Human IAA level in the sampleuse Purified Human IAA antigen to coat microtiter plate wells, make solid-phase antigen, then add IAA antibody to wells, Combined IAA antigen which With HRP labeled ,become antigen – antibody - enzyme-antigen complex, after washing Compley, Add TMB substrate solution, TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of IAA in the samples is then determined by comparing the O.D. of the samples to the standard curve.

                             

                             

                             

                             

                             

                             

                             

                             

                            Materials provided with the kit

                            Materials provided with the kit

                            48determinations

                            96 determinations

                            Storage

                            User manual

                            1

                            1

                             

                            Closure plate membrane

                            2

                            2

                             

                            Sealed bags

                            1

                            1

                             

                            Microelisa stripplate

                            1

                            1

                            2-8

                            Standard180 pmol/L

                            0.5ml×1 bottle

                            0.5ml×1 bottle

                            2-8

                            Standard diluent

                            1.5ml×1 bottle

                            1.5ml×1 bottle

                            2-8

                            HRP-Conjugate reagent

                            3ml×1 bottle

                            6ml×1 bottle

                            2-8

                            Sample diluent

                            3ml×1 bottle

                            6ml×1 bottle

                            2-8

                            Chromogen Solution A

                            3ml×1 bottle

                            6ml×1 bottle

                            2-8

                            Chromogen Solution B

                            3ml×1 bottle

                            6ml×1 bottle

                            2-8

                            Stop Solution

                            3ml×1 bottle

                            6ml×1 bottle

                            2-8

                            wash  solution

                            20ml×20 fold

                            ×1bottle

                            20ml×30 fold

                            ×1bottle

                            2-8

                            Specimen requirements

                            1.       serum- coagulation at room temperature 10-20 minscentrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

                            2.       plasma-use suited EDTA, citrate or heparinized plasma as an anticoagulant,mix 10-20 mins ,centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

                            3.       Urine-collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again. The Operation of Hydrothorax and cerebrospinal fluid Reference to it.

                            4.       cell culture supernatant-detect secretory components, collect sue a sterile container, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant,detect the composition of cells, Dilut cell suspension with PBSPH7.2-7.4, Cell concentration reached 1 million / ml, repeated freeze-thaw cycles, damage cells and release of intracellular components, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant, If precipitation appeared, Centrifugal again.

                            5.       Tissue samples- After cutting samples, check the weight,add PBSPH7.2-7.4, Rapidly frozen with liquid nitrogen, maintain samples at 2-8 after melting,add PBSPH7.4, Homogenized by hand or Grinders, centrifugation 20-min at the speed of 2000-3000 r.p.m. remove supernatant.

                            Assay procedure

                            1.Dilute and add sample to Standard: set 10 Standard wells on the ELISA plates coated, add Standard 100μl to the first and the second well, then add Standard dilution 50μl to the first and the second well, mix; take out 100μl form the first and the second well then add it to the third and the forth well separay. then add Standard dilution 50μl to the third and the forth well ,mix ; then take out 50μl from the third and the forth well discard, add 50μl to the fifth and the sixth well ,then add Standard dilution 50μl to the fifth and the sixth well, mix ; take out 50μl from the fifth and the sixth well and add to the seventh and the eighth well, then add Standard dilution 50μl to the seventh and the eighth well ,mix ; take out 50μl from the seventh and the eighth well and add to the ninth and the tenth well, add Standard dilution 50μl to the ninth and the tenth well, mix , take out 50μl from the ninth and the tenth well discard(add Sample 50μl to each well after Diluting ,(density: 120 pmol/L80 pmol/L 40 pmol/L20 pmol/L10 pmol/L

                            2.add sampleSet blank wells separay (blank comparison wells don’t add sample and HRP-Conjugate reagent, other each step operation is same). testing sample well. add Sample dilution 40μl to testing sample well, then add testing sample 10μl (sample final dilution is 5-fold), add sample to wells , don’t touch the well wall as far as possible, and Gently mix.

                            3.Incubate: After closing plate with Closure plate membrane ,incubate for 30 min at 37.

                            4.Configurate liquid: 30-foldor 20-fold)wash solution diluted 30-fold (or 20-fold) with distilled water and reserve.

                            5.washingUncover Closure plate membrane, discard Liquid, dry by swing, add washing buffer to every well, still for 30s then drain, repeat 5 times, dry by pat.

                            6.add enzymeAdd HRP-Conjugate reagent 50μl to each well, except  blank well.

                            7.incubateOperation with 3.

                            8.washingOperation with 5.

                            9.colorAdd Chromogen Solution A 50ul and Chromogen Solution B to each well, evade the light preservation for 15 min at 37

                            10.Stop the reactionAdd Stop Solution50μl to each well, Stop the reaction(the blue color change to yellow color).

                            11.assaytake blank well as zero , Read absorbance at 450nm after Adding Stop Solution and within 15min.

                            Important notes

                            1.       The kit takes out from the refrigeration environment should be balanced 15-30 minutes in the room temperature, ELISA plates coated if has not use up after opened, the plate should be stored in Sealed bag.

                            2.       washing buffer will Crystallization separation, it can be heated the water helps dissolve when dilute . Washing does not affect the result.

                            3.       add Sample with sampler Each step, And proofread its accuracy frequently, avoids the experimental error. add sample within 5 min, if the number of sample is much , recommend to use Volley .

                            4.       if the testing material content is excessively higher (The sample OD is bigger than the first standard well ),please dilute Sample (n-fold), Please diluente and multiplied by the dilution factor.×n×5.

                            5.       Closure plate membrane only limits the disposable use, to avoid cross-contamination.

                            6.       The substrate evade the light preservation.

                            7.       Please according to use instruction strictly, The test result determination must take the microtiter plate reader as a standard.

                            8.       All samples, washing buffer and each kind of reject should according to infective material process.

                            9.       Do not mix reagents with those from other lots. 

                            Take the standard density as the horizontal, the OD value for the vertical ,draw the standard curve on graph paper, Find out the corresponding density according to the sample OD value by the Sample curve, multiplied by the dilution multiple, or calculate the straight line regression equation of the standard curve with the standard density and the OD value ,with the sample OD value in the equation, calculate the sample density, multiplied by the dilution factor, the result is the sample actual density.

                            Calculate

                            This chartis for reference only

                             

                             


                             

                             

                             

                             

                             

                             

                             

                             

                            Assay range

                            5pmol/L -160 pmol/L

                            Storage and validity

                            1Storage  2-8.

                            2validity six months.



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